| Names | Rank | Age | Date Entered Service | Period of Service | Remarks |
| Mustered in Aug. 21, 1862, at Camp Cleveland, O., by J.R. Paxton, Captain 15th Infantry, U.S.A. Mustered out June 3, 1865, near Washington, C.C., by Jacob Kline, Captain 16th Infantry, U.S.A. | |||||
| Hall, Albert S. | Colonel | 31 | June 3, 1861 | 3 years | Promoted from Lt. Colonel 24th Regiment, O.V.I., Aug. 11, 1862; died July 10, 1863, at Murfreesboro, Tenn. |
| Tolles, William R. | Lt. Colonel | 37 | Aug. 20, 1861 | 3 | Promoted from Captain Co. B, 41st Regiment, O.V.I., to date, Aug. 9, 1862; promoted to Colonel July 19 1863, but not mustered; resigned Jan. 28, 1864. |
| Perkins, George T. | Lt. Colonel | 26 | Aug. 10, 1861 | 3 | Promoted from Major to date July 10, 1863; to Colonel Feb. 18. 1864, but not mustered; brevet Colonel March 13, 1865; mustered out with regiment June 3, 1865. |
| Edwards, Charles G. | Major | 26 | July 23, 1862 | 3 | Promoted from Captain Co. A todate July 10, 1863; to Lt. Colonel Feb. 18, 1864, but not mustered; brevet Lt. Colonel March 13 1865; mustered out with regiment June 3, 1865. |
| Fowler, Charles N. | Surgeon | 34 | Aug. 12, 1862 | 3 | Captured Sept. 20, 1863, at battle of Chickamauga, GA.; mustered out with regiment June 3, 1865. |
| Taft, Harvey S. | As. Surg. | 35 | Aug. 19, 1862 | 3 | Discharged Aug. 10, 1863 |
| Paulding, Joseph G | As. Surg. | 35 | Aug. 19, 1862 | 3 | Resigned April 8, 1863 |
| Turnbull, John | As. Surg. | 23 | June 29, 1863 | 3 | Appointed from civil life; mustered out with regiment June 3, 1865. |
| Robbins, Ambrose M. | Adjutant | 25 | Aug. 1, 1862 | 3 | Resigned March 28, 1863 |
| Dickerman, Albert | Adjutant | 22 | July 28, 1862 | 3 | Promoted to 2d Lt Co. I from Sergt. Major to date Oct. 8, 1862; to 1st Lt. Co. B to date Jan. 21, 1863; appointed Adjutant March 29, 1863; mustered out with regiment June 3, 1865. |
| Wright, Marshall W. | R.Q.M. | 43 | Aug. 1, 1862 | 3 | Resigned April 13, 1864. |
| Van Nostrand, Aaron | Chaplain | 43 | Sept. 26, 1862 | 3 | Died Feb. 27, 1863, at Painesville, O. |
| Morton, Aaron D. | Chaplain | 41 | Sept. 13, 1864 | 3 | Mustered out with regiment June 3, 1865. |
| Butler, Irwin | Ser. Major | 19 | Aug. 9, 1862 | 3 | Promoted from 1st Sergeant Co. C Dec. 16, 1862; to 2d Lt. Co. C Jan. 18, 1863. |
| Watson, Porter | Ser. Major | 21 | Aug. 6, 1862 | 3 | Promoted from Sergeant Co. A Feb. 27, 1863; to 1st Lt. Co. D May 25, 1864. |
| Taylor, Lester D. | Ser. Major | 29 | Aug. 12, 1862 | 3 | Promoted from Sergeant Co. E June 20, 1864; mustered out with regiment June 3, 1865. |
| Smith, Horation M. | Q.M.S | 27 | Aug. 13, 1862 | 3 | Promoted from Private Co. K Aug. 28, 1862; to 2d Lt. Co. K to date Dec. 22, 1862. |
| Cheney, George W. | Q.M.S | 18 | Aug. 4, 1862 | 3 | Promoted from Private Co. G Feb -, 1863; to 2d Lt. Co. K to date Dec. 22, 1862. |
| Gibson, William J. | Com. Ser. | 24 | Aug. 7, 1862 | 3 | Promoted from private Co. G Aug. 20, 1862; mustered out with regiment June 3, 1865. |
| Mehary, John | Hos. St'd | 22 | Aug. 11, 1862 | 3 | Promoted from Sergeant Co. H Oct. 10, 1863; mustered out with regiment June 3, 1865. |
| Rawdon, Horace | Prin. Mus. | 37 | Aug. 9, 1862 | 3 | Promoted from Musician co. I Aug. 31, 1863; returned to company at his own request Sept. 12, 1864. |
| Doty, William | Prin. Mus. | 24 | Jan. 5, 1864 | 3 | Promoted from private Co. F Dec. 23, 1864; transferred as Principal Musician to 38th Regiment, O.V.I, June 1, 1865. |
